Chloroquine lc3-ii

Discussion in 'North West Pharmacy Canada' started by anfox, 26-Feb-2020.

  1. Tirmmiskelm Guest

    Chloroquine lc3-ii


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    Several lysosomal inhibitors such as bafilomycin A1 BafA1, protease inhibitors and chloroquine CQ, have been used interchangeably to block autophagy in in vitro experiments assuming that they all primarily block lysosomal degradation. Autophagy is a natural mechanism in which the cell removes and degrades cellular components with autolysosomes. It is a popular research area because autophagy is related to many physical and pathological processes. In autophagy studies, LC3-I and LC3-II detection is a must-have experiment to track autophagy. Therefore, we would like to share five important notes while quantifying autophagy. Another problem with this method is that LC3-II tends to be much more sensitive to be detected by immunoblotting than LC3-I. Accordingly, simple comparison of LC3-I and LC3-II, or summation of LC3-I and LC3-II for ratio determinations, may not be appropriate, and rather, the amount of LC3-II can be compared between samples.

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    Chloroquine lc3-ii

    What does increasing both LC3 II and p62 mean?, Notes For Autophagy Detection With LC3

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  4. LC3-II turnover is a useful parameter for measuring autophagic flux and can be achieved by analyzing cells in the presence and absence of a lysosomal degradation inhibitor like CQ, which inhibits the fusion of the autophagosomes with the lysosomes.

    • Quantifying autophagy Measuring LC3 puncta and..
    • How to Interpret LC3 Immunoblotting Autophagy Vol 3, No 6.
    • Anti-LC3-I/II Antibody Sigma-Aldrich.

    Chloroquine treatment of cells leads to accumulation of light chain 3-II LC3-II 1-3. This autophagy marker resides within autophagosomal membranes during the autophagic process and is degraded upon fusion with lysosomes. Chloroquine inhibition of these fusion events effectively blocks LC3-II degradation. Molecular Formula C 18 H 26 ClN 3. The correct way to calculate autophagic flux based on the LC3-II band is calculated below. Cells treated with a lysosomal inhibitor, i.e. chloroquine at 40μM for 2hrs, and 4 Cells starved for 16hrs with chloroquine 40μM added for the last 2hrs of treatment. Whole cell lysate from these samples is then loaded onto a 12% polyacrylamide. The expression of LC3-II in the combined treatment was a two-fold increase compared to that in the chloroquine or VNP20009 treatment alone. Hence, VNP20009 induced autophagic flux, which could be.

     
  5. Julia_K XenForo Moderator

    Chloroquine has long been used in the treatment or prevention of malaria from Plasmodium vivax, P. malariae, excluding the malaria parasite Plasmodium falciparum, for it started to develop widespread resistance to it. Chloroquine Oral Uses, Side Effects, Interactions, Pictures. Chloroquine MedlinePlus Drug Information Chloroquine Phosphate HumbleFish Forum
     
  6. utka XenForo Moderator

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